This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0051764
PrimePCR™ PreAmp for SYBR® Green Assay: NTS, Human
PrimePCR™ Template for SYBR® Green Assay: NTS, Human
This gene encodes a common precursor for two peptides neuromedin N and neurotensin. Neurotensin is a secreted tridecapeptide which is widely distributed throughout the central nervous system and may function as a neurotransmitter or a neuromodulator. It may be involved in dopamine-associated pathophysiological events in the maintenance of gut structure and function and in the regulation of fat metabolism. Tissue-specific processing may lead to the formation in some tissues of larger forms of neuromedin N and neurotensin. The large forms may represent more stable peptides that are also biologically active. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.