PrimePCR™ SYBR® Green Assay: SPG20, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0051749

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: SPG20, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: SPG20, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0044487
Assay Design:   exonic
Chromosome Location:   13:36909328-36909462question
Amplicon Length:   105
Splice Variants Targeted:   ENST00000438666 ENST00000494062 ENST00000355182 ENST00000451493

Gene Information

This gene encodes a protein containing a MIT (Microtubule Interacting and Trafficking molecule) domain and is implicated in regulating endosomal trafficking and mitochondria function. The protein localizes to mitochondria and partially co-localizes with microtubules. Stimulation with epidermal growth factor (EGF) results in protein translocation to the plasma membrane and the protein functions in the degradation and intracellular trafficking of EGF receptor. Multiple alternatively spliced variants encoding the same protein have been identified. Mutations associated with this gene cause autosomal recessive spastic paraplegia 20 (Troyer syndrome). [provided by RefSeq Nov 2008]

Gene Symbol:   SPG20
Gene Name:   spastic paraplegia 20 (Troyer syndrome)
Aliases:   KIAA0610, SPARTIN, TAHCCP1
RefSeq:   NC_000013.10 NG_011559.1 NT_024524.14
Ensembl:   ENSG00000133104
Entrez:   23111
Chromosome Mapping:   13q13.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.460000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download