PrimePCR™ SYBR® Green Assay: VSTM2L, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0051732

List Price:    $174.00
Your Price:   Log In
Quantity:  
 
Add to Custom Plate

PrimePCR™ PreAmp for SYBR® Green Assay: VSTM2L, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: VSTM2L, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0044470
Assay Design:   exonic
Chromosome Location:   20:36531860-36560157question
Amplicon Length:   105
Splice Variants Targeted:   ENST00000570896 ENST00000576812 ENST00000311668 ENST00000373461 ENST00000448944 ENST00000373458 ENST00000528712 ENST00000335135 ENST00000522405 ENST00000521807 ENST00000309188 ENST00000290378 ENST00000361200 ENST00000271385 ENST00000443333 ENST00000454744 ENST00000334770 ENST00000409731 ENST00000345517 ENST00000409624 ENST00000369510 ENST00000310260 ENST00000369509 ENST00000355485 ENST00000437198 ENST00000420015 ENST00000341484 ENST00000539279 ENST00000524222 ENST00000351081 ENST00000395125 ENST

Gene Information

Description Not Available

Gene Symbol:   VSTM2L
Gene Name:   V-set and transmembrane domain containing 2 like
Aliases:   C20orf102, dJ1118M15.2
RefSeq:   NC_000020.10 NT_011362.10
Ensembl:   ENSG00000132821
Entrez:   128434
Chromosome Mapping:   20q11.23

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.920000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download