PrimePCR™ SYBR® Green Assay: RAB25, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0051723

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: RAB25, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: RAB25, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0044461
Assay Design:   exonic
Chromosome Location:   1:156040127-156040243question
Amplicon Length:   87
Splice Variants Targeted:   ENST00000361084

Gene Information

RAB proteins such as RAB25 are members of the RAS superfamily of small GTPases that are involved in membrane trafficking. Members of the RAB11 subfamily including RAB25 control the return of internalized membrane-associated moieties to the cell surface (Caswell et al. 2007 [PubMed 17925226]).[supplied by OMIM Aug 2009]

Gene Symbol:   RAB25
Gene Name:   RAB25, member RAS oncogene family
Aliases:   CATX-8, RAB11C
RefSeq:   NC_000001.10 NT_004487.19
Ensembl:   ENSG00000132698
Entrez:   57111
Chromosome Mapping:   1q22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998900
y-intercept 35.340000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download