PrimePCR™ SYBR® Green Assay: INPP5K, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0051696

List Price:    $146.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0044434
Assay Design:   exonic
Chromosome Location:   17:1412999-1416751question
Amplicon Length:   70
Splice Variants Targeted:   ENST00000397335 ENST00000542125 ENST00000498390 ENST00000477910 ENST00000571274 ENST00000575172 ENST00000449479 ENST00000320345 ENST00000406424 ENST00000421807

Gene Information

This gene encodes a protein with 5-phosphatase activity toward polyphosphate inositol. The protein localizes to the cytosol in regions lacking actin stress fibers. It is thought that this protein may negatively regulate the actin cytoskeleton. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq Oct 2008]

Gene Symbol:   INPP5K
Gene Name:   inositol polyphosphate-5-phosphatase K
Aliases:   PPS, SKIP
RefSeq:   NC_000017.10 NG_013017.1 NT_010718.16
Ensembl:   ENSG00000132376
Entrez:   51763
UniGene:   Hs.632238
Chromosome Mapping:   17p13.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 36.290000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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