PrimePCR™ SYBR® Green Assay: ILKAP, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0051692

List Price:    $146.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0044430
Assay Design:   exonic
Chromosome Location:   2:239090738-239092317question
Amplicon Length:   84
Splice Variants Targeted:   ENST00000254654 ENST00000450411

Gene Information

The protein encoded by this gene is a protein serine/threonine phosphatase of the PP2C family. This protein can interact with integrin-linked kinase (ILK/ILK1) a regulator of integrin mediated signaling and regulate the kinase activity of ILK. Through the interaction with ILK this protein may selectively affect the signaling process of ILK-mediated glycogen synthase kinase 3 beta (GSK3beta) and thus participate in Wnt signaling pathway. [provided by RefSeq Jul 2008]

Gene Symbol:   ILKAP
Gene Name:   integrin-linked kinase-associated serine/threonine phosphatase
Aliases:   DKFZp434J2031, FLJ10181, MGC4846, PP2C-DELTA
RefSeq:   NC_000002.11 NT_005120.16
Ensembl:   ENSG00000132323
Entrez:   80895
UniGene:   Hs.92033
Chromosome Mapping:   2q37.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 36.090000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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