PrimePCR™ SYBR® Green Assay: MCCC2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0051675

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0044413
Assay Design:   exonic
Chromosome Location:   5:70928002-70930983question
Amplicon Length:   87
Splice Variants Targeted:   ENST00000575361 ENST00000571852 ENST00000572910 ENST00000340941 ENST00000509358 ENST00000509539 ENST00000323375

Gene Information

This gene encodes the small subunit of 3-methylcrotonyl-CoA carboxylase. This enzyme functions as a heterodimer and catalyzes the carboxylation of 3-methylcrotonyl-CoA to form 3-methylglutaconyl-CoA. Mutations in this gene are associated with 3-Methylcrotonylglycinuria an autosomal recessive disorder of leucine catabolism. [provided by RefSeq Jul 2008]

Gene Symbol:   MCCC2
Gene Name:   methylcrotonoyl-CoA carboxylase 2 (beta)
Aliases:   MCCB
RefSeq:   NC_000005.9 NG_008882.1 NT_006713.15
Ensembl:   ENSG00000131844
Entrez:   64087
UniGene:   Hs.604789
Chromosome Mapping:   5q12-q13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 37.350000
Efficiency 91

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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