PrimePCR™ SYBR® Green Assay: CKMT2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0051664

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0044402
Assay Design:   exonic
Chromosome Location:   5:80548564-80550216question
Amplicon Length:   121
Splice Variants Targeted:   ENST00000374747 ENST00000294485 ENST00000242067 ENST00000350941 ENST00000396127 ENST00000355070 ENST00000354265 ENST00000231498 ENST00000254035 ENST00000511719 ENST00000424301 ENST00000505060 ENST00000437669

Gene Information

Mitochondrial creatine kinase (MtCK) is responsible for the transfer of high energy phosphate from mitochondria to the cytosolic carrier creatine. It belongs to the creatine kinase isoenzyme family. It exists as two isoenzymes sarcomeric MtCK and ubiquitous MtCK encoded by separate genes. Mitochondrial creatine kinase occurs in two different oligomeric forms: dimers and octamers in contrast to the exclusively dimeric cytosolic creatine kinase isoenzymes. Sarcomeric mitochondrial creatine kinase has 80% homology with the coding exons of ubiquitous mitochondrial creatine kinase. This gene contains sequences homologous to several motifs that are shared among some nuclear genes encoding mitochondrial proteins and thus may be essential for the coordinated activation of these genes during mitochondrial biogenesis. Three transcript variants encoding the same protein have been found for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   CKMT2
Gene Name:   creatine kinase, mitochondrial 2 (sarcomeric)
Aliases:   SMTCK
RefSeq:   NC_000005.9 NT_006713.15
Ensembl:   ENSG00000131730
Entrez:   1160
UniGene:   Hs.80691
Chromosome Mapping:   5q13.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 35.940000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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