PrimePCR™ SYBR® Green Assay: HACL1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0051640

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0044378
Assay Design:   exonic
Chromosome Location:   3:15637930-15642895question
Amplicon Length:   83
Splice Variants Targeted:   ENST00000414979 ENST00000421993 ENST00000457447 ENST00000456194 ENST00000451445 ENST00000435217 ENST00000321169

Gene Information

Description Not Available

Gene Symbol:   HACL1
Gene Name:   2-hydroxyacyl-CoA lyase 1
Aliases:   2-HPCL, FLJ55041, HPCL, HPCL2, PHYH2
RefSeq:   NC_000003.11 NG_008019.1 NT_022517.18
Ensembl:   ENSG00000131373
Entrez:   26061
UniGene:   Hs.63290
Chromosome Mapping:   3p25.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 36.340000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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