PrimePCR™ SYBR® Green Assay: ZNF227, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0051625

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0044363
Assay Design:   exonic
Chromosome Location:   19:44717252-44717392question
Amplicon Length:   111
Splice Variants Targeted:   ENST00000586228 ENST00000588219 ENST00000313040 ENST00000589707 ENST00000588394 ENST00000589005 ENST00000586048 ENST00000391961

Gene Information

Description Not Available

Gene Symbol:   ZNF227
Gene Name:   zinc finger protein 227
Aliases:   Not Available
RefSeq:   NC_000019.9 NT_011109.16
Ensembl:   ENSG00000131115
Entrez:   7770
UniGene:   Hs.298089
Chromosome Mapping:   -

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 37.370000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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