PrimePCR™ SYBR® Green Assay: LAMA5, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0051592

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: LAMA5, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: LAMA5, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0044330
Assay Design:   exonic
Chromosome Location:   20:60921625-60921830question
Amplicon Length:   91
Splice Variants Targeted:   ENST00000252999 ENST00000370677 ENST00000370692

Gene Information

Components of the extracellular matrix exert myriad effects on tissues throughout the body. In particular the laminins a family of heterotrimeric extracellular glycoproteins affect tissue development and integrity in such diverse organs as the kidney lung skin and nervous system. It is thought that laminins mediate the attachment migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. Laminins function as heterotrimeric complexes of alpha beta and gamma chains with each chain type representing a different subfamily of proteins. The protein encoded by this gene belongs to the alpha subfamily of laminin chains and is a major component of basement membranes. Two transcript variants encoding different isoforms have been found for this gene but the full-length nature of one of them has not been determined. [provided by RefSeq Jul 2008]

Gene Symbol:   LAMA5
Gene Name:   laminin, alpha 5
Aliases:   KIAA1907
RefSeq:   NC_000020.10 NT_011362.10
Ensembl:   ENSG00000130702
Entrez:   3911
Chromosome Mapping:   20q13.2-q13.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 1.000000
y-intercept 36.030000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download