PrimePCR™ SYBR® Green Assay: ZNF337, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0051590

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: ZNF337, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: ZNF337, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0044328
Assay Design:   exonic
Chromosome Location:   20:25656200-25656347question
Amplicon Length:   118
Splice Variants Targeted:   ENST00000278772 ENST00000353796 ENST00000396912 ENST00000377671 ENST00000316358 ENST00000396026 ENST00000401790 ENST00000402618 ENST00000538547 ENST00000535822 ENST00000458705 ENST00000432900 ENST00000374618 ENST00000307441 ENST00000359467 ENST00000399484 ENST00000544825 ENST00000401847 ENST00000328239 ENST00000542006 ENST00000354698 ENST00000400845 ENST00000422325 ENST00000478296 ENST00000376436 ENST00000252979 ENST00000538750

Gene Information

Description Not Available

Gene Symbol:   ZNF337
Gene Name:   zinc finger protein 337
Aliases:   Not Available
RefSeq:   NC_000020.10 NT_011387.8
Ensembl:   ENSG00000130684
Entrez:   26152
Chromosome Mapping:   20p11.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 36.550000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download