This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0051582
PrimePCR™ PreAmp for SYBR® Green Assay: TRPM4, Human
PrimePCR™ Template for SYBR® Green Assay: TRPM4, Human
The protein encoded by this gene is a calcium-activated nonselective ion channel that mediates transport of monovalent cations across membranes thereby depolarizing the membrane. The activity of the encoded protein increases with increasing intracellular calcium concentration but this channel does not transport calcium. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Aug 2010]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.