PrimePCR™ SYBR® Green Assay: APOC1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0051561

List Price:    $146.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0044299
Assay Design:   exonic
Chromosome Location:   19:45418199-45419529question
Amplicon Length:   61
Splice Variants Targeted:   ENST00000298466 ENST00000425225 ENST00000588750 ENST00000588802 ENST00000592535 ENST00000592885 ENST00000589078 ENST00000586638 ENST00000252491 ENST00000373468 ENST00000055682

Gene Information

The protein encoded by this gene is a member of the apolipoprotein C1 family. This gene is expressed primarily in the liver and it is activated when monocytes differentiate into macrophages. A pseudogene of this gene is located 4 kb downstream in the same orientation on the same chromosome. This gene is mapped to chromosome 19 where it resides within a apolipoprotein gene cluster. Alternatively spliced transcript variants have been found for this gene but the biological validity of some variants has not been determined. [provided by RefSeq Jul 2008]

Gene Symbol:   APOC1
Gene Name:   apolipoprotein C-I
Aliases:   Not Available
RefSeq:   NC_000019.9 NG_012859.1 NT_011109.16
Ensembl:   ENSG00000130208
Entrez:   341
UniGene:   Hs.110675
Chromosome Mapping:   19q13.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.920000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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