PrimePCR™ SYBR® Green Assay: TOMM40, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0051560

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0044298
Assay Design:   exonic
Chromosome Location:   19:45397135-45397309question
Amplicon Length:   66
Splice Variants Targeted:   ENST00000259235 ENST00000259234 ENST00000424298 ENST00000357702 ENST00000252487 ENST00000592434 ENST00000426677 ENST00000589649 ENST00000405636 ENST00000584137 ENST00000362042 ENST00000585291 ENST00000357480 ENST00000369393 ENST00000428876

Gene Information

TOMM40 is the channel-forming subunit of the translocase of the mitochondrial outer membrane (TOM) complex that is essential for protein import into mitochondria (Humphries et al. 2005 [PubMed 15644312]).[supplied by OMIM May 2008]

Gene Symbol:   TOMM40
Gene Name:   translocase of outer mitochondrial membrane 40 homolog (yeast)
Aliases:   C19orf1, D19S1177E, PER-EC1, PEREC1, TOM40
RefSeq:   NC_000019.9 NG_007084.2 NT_011109.16
Ensembl:   ENSG00000130204
Entrez:   10452
UniGene:   Hs.655909
Chromosome Mapping:   19q13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.960000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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