PrimePCR™ SYBR® Green Assay: GALNT8, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0051552

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0044290
Assay Design:   exonic
Chromosome Location:   12:4873192-4874624question
Amplicon Length:   72
Splice Variants Targeted:   ENST00000252318 ENST00000542998 ENST00000535354

Gene Information

This gene encodes a member of the UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase (GalNAc-T) family of enzymes. GalNAc-Ts initiate mucin-type O-linked glycosylation in the Golgi apparatus by catalyzing the transfer of GalNAc to serine and threonine residues on target proteins. They are characterized by an N-terminal transmembrane domain a stem region a lumenal catalytic domain containing a GT1 motif and Gal/GalNAc transferase motif and a C-terminal ricin/lectin-like domain. GalNAc-Ts have different but overlapping substrate specificities and patterns of expression. [provided by RefSeq Jul 2008]

Gene Symbol:   GALNT8
Gene Name:   UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 8 (GalNAc-T8)
Aliases:   FLJ17364, GALNAC-T8
RefSeq:   NC_000012.11 NT_009759.16
Ensembl:   ENSG00000130035
Entrez:   26290
UniGene:   Hs.511985
Chromosome Mapping:   12p13.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 35.970000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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