PrimePCR™ SYBR® Green Assay: RPAIN, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0051518

List Price:    $138.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0044256
Assay Design:   exonic
Chromosome Location:   17:5324673-5324780question
Amplicon Length:   78
Splice Variants Targeted:   ENST00000536255 ENST00000381209 ENST00000381208 ENST00000327154 ENST00000405578 ENST00000574003

Gene Information

Description Not Available

Gene Symbol:   RPAIN
Gene Name:   RPA interacting protein
Aliases:   FLJ25625, FLJ30490, FLJ42429, HRIP, MGC4189, RIP
RefSeq:   NC_000017.10 NT_010718.16
Ensembl:   ENSG00000129197
Entrez:   84268
UniGene:   Hs.462086
Chromosome Mapping:   17p13.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.680000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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