PrimePCR™ SYBR® Green Assay: RFPL1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0051475

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0044213
Assay Design:   exonic
Chromosome Location:   22:29838260-29838379question
Amplicon Length:   90
Splice Variants Targeted:   ENST00000400237 ENST00000248980 ENST00000248983 ENST00000400236 ENST00000397468 ENST00000249007 ENST00000382088 ENST00000354373

Gene Information

Description Not Available

Gene Symbol:   RFPL1
Gene Name:   ret finger protein-like 1
Aliases:   MGC132428, RNF78
RefSeq:   NC_000022.10 NT_011520.12
Ensembl:   ENSG00000128250
Entrez:   5988
UniGene:   Hs.648249
Chromosome Mapping:   22q12.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 35.810000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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