PrimePCR™ SYBR® Green Assay: DGCR6L, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0051471

List Price:    $146.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0044209
Assay Design:   exonic
Chromosome Location:   22:20302906-20302998question
Amplicon Length:   63
Splice Variants Targeted:   ENST00000582970 ENST00000508628 ENST00000263650 ENST00000389989 ENST00000525498 ENST00000348039 ENST00000301178 ENST00000359092 ENST00000413981 ENST00000331444 ENST00000222139 ENST00000592375 ENST00000268876 ENST00000394570 ENST00000591048 ENST00000433649 ENST00000378449 ENST00000439287 ENST00000300515 ENST00000559949 ENST00000557886 ENST00000561062 ENST00000358583 ENST00000397256 ENST00000426895 ENST00000310252 ENST00000443148 ENST00000383827 ENST00000455274 ENST00000430793 ENST00000547186 ENST

Gene Information

This gene the result of a duplication at this locus is one of two functional genes encoding nearly identical proteins that have similar expression patterns. The product of this gene is a protein that shares homology with the Drosophila gonadal protein expressed in gonadal tissues and germ cells and with the human laminin gamma-1 chain that functions in cell attachment and migration. This gene is located in a region of chromosome 22 implicated in the DiGeorge syndrome one facet of a broader collection of anomalies referred to as the CATCH 22 syndrome. [provided by RefSeq Jul 2008]

Gene Symbol:   DGCR6L
Gene Name:   DiGeorge syndrome critical region gene 6-like
Aliases:   FLJ10666
RefSeq:   NC_000022.10 NT_011519.10
Ensembl:   ENSG00000128185
Entrez:   85359
UniGene:   Hs.410965
Chromosome Mapping:   22q11

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 36.200000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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