PrimePCR™ SYBR® Green Assay: PEX1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0051460

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0044198
Assay Design:   exonic
Chromosome Location:   7:92131385-92132462question
Amplicon Length:   87
Splice Variants Targeted:   ENST00000438045 ENST00000248633 ENST00000428214 ENST00000541751

Gene Information

This gene encodes a member of the AAA ATPase family a large group of ATPases associated with diverse cellular activities. This protein is cytoplasmic but is often anchored to a peroxisomal membrane where it forms a heteromeric complex and plays a role in the import of proteins into peroxisomes and peroxisome biogenesis. Mutations in this gene have been associated with complementation group 1 peroxisomal disorders such as neonatal adrenoleukodystrophy infantile Refsum disease and Zellweger syndrome. [provided by RefSeq Jul 2008]

Gene Symbol:   PEX1
Gene Name:   peroxisomal biogenesis factor 1
Aliases:   ZWS, ZWS1
RefSeq:   NC_000007.13 NG_008341.1 NT_007933.15
Ensembl:   ENSG00000127980
Entrez:   5189
UniGene:   Hs.164682
Chromosome Mapping:   7q21.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 36.870000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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