PrimePCR™ SYBR® Green Assay: AAMP, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0051454

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0044192
Assay Design:   exonic
Chromosome Location:   2:219131690-219132318question
Amplicon Length:   92
Splice Variants Targeted:   ENST00000248450 ENST00000444053 ENST00000420660 ENST00000447885

Gene Information

The gene product is an immunoglobulin-type protein. It is found to be expressed strongly in endothelial cells cytotrophoblasts and poorly differentiated colon adenocarcinoma cells found in lymphatics. The protein contains a heparin-binding domain and mediates heparin-sensitive cell adhesion. [provided by RefSeq Jul 2008]

Gene Symbol:   AAMP
Gene Name:   angio-associated, migratory cell protein
Aliases:   Not Available
RefSeq:   NC_000002.11 NG_017060.1 NT_005403.17
Ensembl:   ENSG00000127837
Entrez:   14
UniGene:   Hs.83347
Chromosome Mapping:   2q35

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 1.000000
y-intercept 35.950000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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