PrimePCR™ SYBR® Green Assay: GNG13, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0051450

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: GNG13, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: GNG13, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0044188
Assay Design:   exonic
Chromosome Location:   16:848982-849082question
Amplicon Length:   71
Splice Variants Targeted:   ENST00000248150 ENST00000262593 ENST00000395939 ENST00000404767 ENST00000389470 ENST00000342620 ENST00000361221 ENST00000375415 ENST00000452522 ENST00000434513

Gene Information

Heterotrimeric G proteins which consist of alpha (see MIM 139320) beta (see MIM 139380) and gamma subunits function as signal transducers for the 7-transmembrane-helix G protein-coupled receptors. GNG13 is a gamma subunit that is expressed in taste retinal and neuronal tissues and plays a key role in taste transduction (Li et al. 2006 [PubMed 16473877]).[supplied by OMIM Oct 2009]

Gene Symbol:   GNG13
Gene Name:   guanine nucleotide binding protein (G protein), gamma 13
Aliases:   G(gamma)13, h2-35
RefSeq:   NC_000016.9 NT_010393.16
Ensembl:   ENSG00000127588
Entrez:   51764
Chromosome Mapping:   16p13.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997600
y-intercept 35.260000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download