This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCEP0051441
PrimePCR™ PreAmp for SYBR® Green Assay: PTPRB, Human
PrimePCR™ Template for SYBR® Green Assay: PTPRB, Human
The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth differentiation mitotic cycle and oncogenic transformation. This PTP contains an extracellular domain a single transmembrane segment and one intracytoplasmic catalytic domain thus belongs to receptor type PTP. The extracellular region of this PTP is composed of multiple fibronectin type_III repeats which was shown to interact with neuronal receptor and cell adhesion molecules such as contactin and tenascin C. This protein was also found to interact with sodium channels and thus may regulate sodium channels by altering tyrosine phosphorylation status. The functions of the interaction partners of this protein implicate the roles of this PTP in cell adhesion neurite growth and neuronal differentiation. Alternate transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq May 2011]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.