PrimePCR™ SYBR® Green Assay: COX7C, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0051437

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: COX7C, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: COX7C, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0044175
Assay Design:   exonic
Chromosome Location:   5:85913844-85913944question
Amplicon Length:   71
Splice Variants Targeted:   ENST00000409299 ENST00000344022 ENST00000328333 ENST00000454817 ENST00000270357 ENST00000437406 ENST00000301067 ENST00000339615 ENST00000438251 ENST00000323703 ENST00000407879 ENST00000247655 ENST00000509578 ENST00000515763

Gene Information

Cytochrome c oxidase (COX) the terminal component of the mitochondrial respiratory chain catalyzes the electron transfer from reduced cytochrome c to oxygen. This component is a heteromeric complex consisting of 3 catalytic subunits encoded by mitochondrial genes and multiple structural subunits encoded by nuclear genes. The mitochondrially-encoded subunits function in electron transfer and the nuclear-encoded subunits may function in the regulation and assembly of the complex. This nuclear gene encodes subunit VIIc which shares 87% and 85% amino acid sequence identity with mouse and bovine COX VIIc respectively and is found in all tissues. A pseudogene COX7CP1 has been found on chromosome 13. [provided by RefSeq Jul 2008]

Gene Symbol:   COX7C
Gene Name:   cytochrome c oxidase subunit VIIc
Aliases:   Not Available
RefSeq:   NC_000005.9 NT_006713.15
Ensembl:   ENSG00000127184
Entrez:   1350
Chromosome Mapping:   5q14

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 36.510000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download