PrimePCR™ SYBR® Green Assay: RGS13, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0051432

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0044170
Assay Design:   exonic
Chromosome Location:   1:192628626-192628773question
Amplicon Length:   118
Splice Variants Targeted:   ENST00000391995 ENST00000543215

Gene Information

The protein encoded by this gene is a member of the regulator of G protein signaling (RGS) family. RGS family members share similarity with S. cerevisiae SST2 and C. elegans egl-10 proteins which contain a characteristic conserved RGS domain. RGS proteins accelerate GTPase activity of G protein alpha-subunits thereby driving G protein into their inactive GDP-bound form thus negatively regulating G protein signaling. RGS proteins have been implicated in the fine tuning of a variety of cellular events in response to G protein-coupled receptor activation. The biological function of this gene however is unknown. Two transcript variants encoding the same isoform exist. [provided by RefSeq Jul 2008]

Gene Symbol:   RGS13
Gene Name:   regulator of G-protein signaling 13
Aliases:   MGC17173
RefSeq:   NC_000001.10 NT_004487.19
Ensembl:   ENSG00000127074
Entrez:   6003
UniGene:   Hs.497220
Chromosome Mapping:   1q31.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999000
y-intercept 36.660000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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