This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCEP0051319
PrimePCR™ PreAmp for SYBR® Green Assay: MED20, Human
PrimePCR™ Template for SYBR® Green Assay: MED20, Human
MED20 is a subunit of the Mediator complex a multiprotein coactivator of RNA transcription that interacts with DNA-bound transcriptional activators RNA polymerase II (see MIM 180660) and general initiation factors (Sato et al. 2003 [PubMed 14576168]).[supplied by OMIM Aug 2009]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.