PrimePCR™ SYBR® Green Assay: MED20, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0051319

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: MED20, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: MED20, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0044057
Assay Design:   exonic
Chromosome Location:   6:41877097-41877204question
Amplicon Length:   78
Splice Variants Targeted:   ENST00000265350 ENST00000409312 ENST00000394251 ENST00000409060

Gene Information

MED20 is a subunit of the Mediator complex a multiprotein coactivator of RNA transcription that interacts with DNA-bound transcriptional activators RNA polymerase II (see MIM 180660) and general initiation factors (Sato et al. 2003 [PubMed 14576168]).[supplied by OMIM Aug 2009]

Gene Symbol:   MED20
Gene Name:   mediator complex subunit 20
Aliases:   DKFZp586D2223, MGC29869, PRO0213, TRFP
RefSeq:   NC_000006.11 NT_007592.15
Ensembl:   ENSG00000124641
Entrez:   9477
Chromosome Mapping:   6p21.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 37.110000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download