PrimePCR™ SYBR® Green Assay: IQSEC2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0051298

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: IQSEC2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: IQSEC2, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0044036
Assay Design:   exonic
Chromosome Location:   X:53279919-53280067question
Amplicon Length:   119
Splice Variants Targeted:   ENST00000264819 ENST00000396435 ENST00000375365 ENST00000375368 ENST00000278550 ENST00000297440 ENST00000440747 ENST00000313147 ENST00000291503 ENST00000357324 ENST00000587518 ENST00000053867 ENST00000588143 ENST00000593167 ENST00000591740 ENST00000592783 ENST00000587387 ENST00000589265 ENST00000323205 ENST00000248924 ENST00000596391 ENST00000600562 ENST00000602247

Gene Information

This gene encodes a guanine nucleotide exchange factor for the ARF family of small GTP-binding proteins. The encoded protein is a component of the postsynaptic density at excitatory synapses and may play a critical role in cytoskeletal and synaptic organization through the activation of selected ARF substrates including ARF1 and ARF6. Mutations in this gene have been implicated in nonsyndromic X-linked mental retardation. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq Aug 2011]

Gene Symbol:   IQSEC2
Gene Name:   IQ motif and Sec7 domain 2
Aliases:   BRAG1, KIAA0522, MRX1
RefSeq:   NC_000023.10 NG_021296.1 NT_011630.14
Ensembl:   ENSG00000124313
Entrez:   23096
Chromosome Mapping:   Xp11.22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 36.620000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download