PrimePCR™ SYBR® Green Assay: EXOSC9, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0051263

List Price:    $146.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0044001
Assay Design:   exonic
Chromosome Location:   4:122731128-122731249question
Amplicon Length:   92
Splice Variants Targeted:   ENST00000243498 ENST00000379663 ENST00000509800 ENST00000512454 ENST00000511132

Gene Information

This gene encodes a component of the human exosome a exoribonuclease complex which processes and degrades RNA in the nucleus and cytoplasm. This component may play a role in mRNA degradation and the polymyositis/scleroderma autoantigen complex. Alternative splicing results in multiple transcript variants. [provided by RefSeq Aug 2011]

Gene Symbol:   EXOSC9
Gene Name:   exosome component 9
Aliases:   PM/Scl-75, PMSCL1, RRP45, Rrp45p, p5, p6
RefSeq:   NC_000004.11 NT_016354.19
Ensembl:   ENSG00000123737
Entrez:   5393
UniGene:   Hs.91728
Chromosome Mapping:   4q27

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 36.260000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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