PrimePCR™ SYBR® Green Assay: SLC36A1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0051261

List Price:    $146.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0043999
Assay Design:   exonic
Chromosome Location:   5:150838380-150843141question
Amplicon Length:   115
Splice Variants Targeted:   ENST00000520111 ENST00000520701 ENST00000429484 ENST00000243389 ENST00000517945 ENST00000521925 ENST00000519829

Gene Information

This gene encodes a member of the eukaryote-specific amino acid/auxin permease (AAAP) 1 transporter family. The encoded protein functions as a proton-dependent small amino acid transporter. This gene is clustered with related family members on chromosome 5q33.1. [provided by RefSeq Jul 2008]

Gene Symbol:   SLC36A1
Gene Name:   solute carrier family 36 (proton/amino acid symporter), member 1
Aliases:   Dct1, LYAAT1, PAT1, TRAMD3
RefSeq:   NC_000005.9 NT_029289.11
Ensembl:   ENSG00000123643
Entrez:   206358
UniGene:   Hs.269004
Chromosome Mapping:   5q33.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 36.780000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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