PrimePCR™ SYBR® Green Assay: COL10A1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0051254

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: COL10A1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: COL10A1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0043992
Assay Design:   exonic
Chromosome Location:   6:116446518-116446625question
Amplicon Length:   78
Splice Variants Targeted:   ENST00000327673 ENST00000452729 ENST00000418500 ENST00000243222

Gene Information

This gene encodes the alpha chain of type X collagen a short chain collagen expressed by hypertrophic chondrocytes during endochondral ossification. Unlike type VIII collagen the other short chain collagen type X collagen is a homotrimer. Mutations in this gene are associated with Schmid type metaphyseal chondrodysplasia (SMCD) and Japanese type spondylometaphyseal dysplasia (SMD). [provided by RefSeq Jul 2008]

Gene Symbol:   COL10A1
Gene Name:   collagen, type X, alpha 1
Aliases:   Not Available
RefSeq:   NC_000006.11 NG_008032.1 NG_021351.1 NT_025741.15
Ensembl:   ENSG00000123500
Entrez:   1300
Chromosome Mapping:   6q21-q22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 37.050000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download