PrimePCR™ SYBR® Green Assay: METTL21B, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0051249

List Price:    $142.00
Your Price:   Log In
 
Add to Custom Plate

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0043987
Assay Design:   exonic
Chromosome Location:   12:58174412-58174542question
Amplicon Length:   101
Splice Variants Targeted:   ENST00000551420 ENST00000300209 ENST00000333012

Gene Information

Description Not Available

Gene Symbol:   METTL21B
Gene Name:   methyltransferase like 21B
Aliases:   DKFZp586D0919, FAM119B
RefSeq:   NC_000012.11 NG_016971.1 NT_029419.12
Ensembl:   ENSG00000123427
Entrez:   25895
UniGene:   Hs.632720
Chromosome Mapping:   12q14.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 36.970000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download