PrimePCR™ SYBR® Green Assay: NUDT10, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0051227

List Price:    $138.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0043965
Assay Design:   exonic
Chromosome Location:   X:51079143-51079291question
Amplicon Length:   119
Splice Variants Targeted:   ENST00000376006 ENST00000356450 ENST00000598821 ENST00000594895

Gene Information

NUDT10 belongs to a subgroup of phosphohydrolases that preferentially attack diphosphoinositol polyphosphates (Hidaka et al. 2002 [PubMed 12105228]).[supplied by OMIM Mar 2008]

Gene Symbol:   NUDT10
Gene Name:   nudix (nucleoside diphosphate linked moiety X)-type motif 10
Aliases:   APS2, DIPP3a, hDIPP3alpha
RefSeq:   NC_000023.10 NT_011638.13
Ensembl:   ENSG00000122824
Entrez:   170685
UniGene:   Hs.375178
Chromosome Mapping:   Xp11.23

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.720000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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