This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCEP0051224
PrimePCR™ PreAmp for SYBR® Green Assay: DNAI1, Human
PrimePCR™ Template for SYBR® Green Assay: DNAI1, Human
The inner- and outer-arm dyneins which bridge between the doublet microtubules in axonemes are the force-generating proteins responsible for the sliding movement in axonemes. The intermediate and light chains thought to form the base of the dynein arm help mediate attachment and may also participate in regulating dynein activity. This gene encodes an intermediate chain dynein belonging to the large family of motor proteins. Mutations in this gene result in abnormal ciliary ultrastructure and function associated with primary ciliary dyskinesia (PCD) and Kartagener syndrome. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.