PrimePCR™ SYBR® Green Assay: FKBP9, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0051216

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0043954
Assay Design:   exonic
Chromosome Location:   7:33035814-33035904question
Amplicon Length:   61
Splice Variants Targeted:   ENST00000575445 ENST00000423312 ENST00000315264 ENST00000266031 ENST00000218758 ENST00000592828 ENST00000503128 ENST00000398261 ENST00000228606 ENST00000373095 ENST00000373084 ENST00000242209 ENST00000418354 ENST00000538336 ENST00000538443 ENST00000490776 ENST00000395527 ENST00000261503 ENST00000395530 ENST00000581399 ENST00000536879

Gene Information

Description Not Available

Gene Symbol:   FKBP9
Gene Name:   FK506 binding protein 9, 63 kDa
Aliases:   DKFZp586B1723, FKBP60, FKBP63, MGC126772, MGC138258, PPIase
RefSeq:   NC_000007.13 NT_007819.17
Ensembl:   ENSG00000122642
Entrez:   11328
UniGene:   Hs.103934
Chromosome Mapping:   7p11.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 36.010000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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