PrimePCR™ SYBR® Green Assay: NPY, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0051215

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: NPY, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: NPY, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0043953
Assay Design:   exonic
Chromosome Location:   7:24329198-24331419question
Amplicon Length:   109
Splice Variants Targeted:   ENST00000242152 ENST00000407573 ENST00000405982

Gene Information

This gene encodes a neuropeptide that is widely expressed in the central nervous system and influences many physiological processes including cortical excitability stress response food intake circadian rhythms and cardiovascular function. The neuropeptide functions through G protein-coupled receptors to inhibit adenylyl cyclase activate mitogen-activated protein kinase (MAPK) regulate intracellular calcium levels and activate potassium channels. A polymorphism in this gene resulting in a change of leucine 7 to proline in the signal peptide is associated with elevated cholesterol levels higher alcohol consumption and may be a risk factor for various metabolic and cardiovascular diseases. [provided by RefSeq Nov 2009]

Gene Symbol:   NPY
Gene Name:   neuropeptide Y
Aliases:   PYY4
RefSeq:   NC_000007.13 NG_016148.1 NT_007819.17
Ensembl:   ENSG00000122585
Entrez:   4852
Chromosome Mapping:   7p15.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.900000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download