This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCEP0051163
PrimePCR™ PreAmp for SYBR® Green Assay: PSPC1, Human
PrimePCR™ Template for SYBR® Green Assay: PSPC1, Human
This gene encodes a nucleolar protein that localizes to punctate subnuclear structures that occur close to splicing speckles known as paraspeckles. These paraspeckles are composed of RNA-protein structures that include a non-coding RNA NEAT1/Men epsilon/beta and the Drosophila Behavior Human Splicing family of proteins which include the product of this gene and the P54NRB/NONO and PSF/SFPQ proteins. Paraspeckles may function in the control of gene expression via an RNA nuclear retention mechanism. The protein encoded by this gene is found in paraspeckles in transcriptionally active cells but it localizes to unique cap structures at the nucleolar periphery when RNA polymerase II transcription is inhibited or during telophase. Alternative splicing of this gene results in multiple transcript variants. A related pseudogene which is also located on chromosome 13 has been identified. [provided by RefSeq Aug 2011]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.