PrimePCR™ SYBR® Green Assay: ZFP30, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0051137

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0043875
Assay Design:   exonic
Chromosome Location:   19:38134261-38138728question
Amplicon Length:   115
Splice Variants Targeted:   ENST00000588268 ENST00000360357 ENST00000592829 ENST00000356725 ENST00000528994 ENST00000586323 ENST00000525288 ENST00000527645 ENST00000587143 ENST00000586138 ENST00000588578 ENST00000587986 ENST00000589319 ENST00000587490 ENST00000542455 ENST00000445217 ENST00000592533 ENST00000589117 ENST00000316433 ENST00000590588 ENST00000586134 ENST00000343599 ENST00000589018 ENST00000351218 ENST00000514101 ENST00000587809 ENST00000588979 ENST00000392144 ENST00000589143 ENST00000301310 ENST00000586929 ENST

Gene Information

Description Not Available

Gene Symbol:   ZFP30
Gene Name:   zinc finger protein 30 homolog (mouse)
Aliases:   KIAA0961, ZNF745
RefSeq:   NC_000019.9 NT_011109.16
Ensembl:   ENSG00000120784
Entrez:   22835
UniGene:   Hs.716719
Chromosome Mapping:   19q13.12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.996200
y-intercept 35.410000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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