PrimePCR™ SYBR® Green Assay: GORAB, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0051119

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0043857
Assay Design:   exonic
Chromosome Location:   1:170508589-170508699question
Amplicon Length:   81
Splice Variants Targeted:   ENST00000367763 ENST00000367762

Gene Information

This gene encodes a member of the golgin family a group of coiled-coil proteins localized to the Golgi. The encoded protein may function in the secretory pathway. The encoded protein which also localizes to the cytoplasm was identified by interactions with the N-terminal kinase-like protein and thus it may function in mitosis. Mutations in this gene have been associated with geroderma osteodysplastica. Alternatively spliced transcript variants have been described. [provided by RefSeq Mar 2009]

Gene Symbol:   GORAB
Gene Name:   golgin, RAB6-interacting
Aliases:   FLJ11752, GO, MGC51263, MGC70512, NTKLBP1, SCYL1BP1
RefSeq:   NC_000001.10 NT_004487.19 NG_012237.1
Ensembl:   ENSG00000120370
Entrez:   92344
UniGene:   Hs.183702
Chromosome Mapping:   1q24.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 35.940000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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