PrimePCR™ SYBR® Green Assay: TNFSF18, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0051118

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0043856
Assay Design:   exonic
Chromosome Location:   1:173010393-173010525question
Amplicon Length:   103
Splice Variants Targeted:   ENST00000404377 ENST00000239468

Gene Information

The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for receptor TNFRSF18/AITR/GITR. It has been shown to modulate T lymphocyte survival in peripheral tissues. This cytokine is also found to be expressed in endothelial cells and is thought to be important for interaction between T lymphocytes and endothelial cells. [provided by RefSeq Jul 2008]

Gene Symbol:   TNFSF18
Gene Name:   tumor necrosis factor (ligand) superfamily, member 18
Aliases:   AITRL, GITRL, MGC138237, TL6, hGITRL
RefSeq:   NC_000001.10 NT_004487.19
Ensembl:   ENSG00000120337
Entrez:   8995
UniGene:   Hs.248197
Chromosome Mapping:   1q23

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.910000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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