This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCEP0051093
PrimePCR™ PreAmp for SYBR® Green Assay: PPP1R3C, Human
PrimePCR™ Template for SYBR® Green Assay: PPP1R3C, Human
This gene encodes a regulatory subunit of protein phosphatase-1 (PP1). PP1 catalyzes reversible protein phosphorylation which is important in a wide range of cellular activities: neuronal muscular RNA splicing protein synthesis cell death and glycogen metabolism to name just a few. By interacting with different regulatory subunits PP1 is directed to different parts of the cell to different substrates or to respond to extracellular signals. [provided by RefSeq Oct 2011]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.