PrimePCR™ SYBR® Green Assay: ACOT2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0051081

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0043819
Assay Design:   exonic
Chromosome Location:   14:74036001-74036091question
Amplicon Length:   61
Splice Variants Targeted:   ENST00000538782 ENST00000238651 ENST00000447388 ENST00000425457 ENST00000456393 ENST00000372654 ENST00000372653 ENST00000372669 ENST00000372652 ENST00000372651 ENST00000440226 ENST00000416859 ENST00000308733

Gene Information

This gene encodes a member of the acyl-CoA thioesterase protein family and is one of four acyl-CoA hydrolase genes located in a cluster on chromosome 14. Alternative splicing results in multiple transcript variants. [provided by RefSeq Jan 2012]

Gene Symbol:   ACOT2
Gene Name:   acyl-CoA thioesterase 2
Aliases:   CTE1A, MTE1, PTE2, PTE2A, ZAP128
RefSeq:   NC_000014.8 NT_026437.12
Ensembl:   ENSG00000119673
Entrez:   10965
UniGene:   Hs.446685
Chromosome Mapping:   14q24.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 35.950000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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