PrimePCR™ SYBR® Green Assay: ONECUT2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0051074

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: ONECUT2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: ONECUT2, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0043812
Assay Design:   exonic
Chromosome Location:   18:55143686-55143827question
Amplicon Length:   112
Splice Variants Targeted:   ENST00000491143 ENST00000481727 ENST00000262095

Gene Information

This gene encodes a member of the onecut family of transcription factors which are characterized by a cut domain and an atypical homeodomain. The protein binds to specific DNA sequences and stimulates expression of target genes including genes involved in melanocyte and hepatocyte differentiation. [provided by RefSeq Jul 2008]

Gene Symbol:   ONECUT2
Gene Name:   one cut homeobox 2
Aliases:   MGC120377, MGC120378, OC-2, OC2
RefSeq:   NC_000018.9 NT_025028.14
Ensembl:   ENSG00000119547
Entrez:   9480
Chromosome Mapping:   18q21.31

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 35.880000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download