PrimePCR™ SYBR® Green Assay: NEK6, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0051071

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: NEK6, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: NEK6, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0043809
Assay Design:   exonic
Chromosome Location:   9:127076255-127083834question
Amplicon Length:   77
Splice Variants Targeted:   ENST00000373603 ENST00000373600 ENST00000320246 ENST00000444973 ENST00000454453 ENST00000373596 ENST00000425237 ENST00000423785 ENST00000422297 ENST00000447379 ENST00000540326 ENST00000539416 ENST00000394199 ENST00000545174 ENST00000546191

Gene Information

The protein encoded by this gene is a kinase required for progression through the metaphase portion of mitosis. Inhibition of the encoded protein can lead to apoptosis. This protein also can enhance tumorigenesis by suppressing tumor cell senescence. Several transcript variants encoding a few different isoforms have been found for this gene. [provided by RefSeq Oct 2011]

Gene Symbol:   NEK6
Gene Name:   NIMA (never in mitosis gene a)-related kinase 6
Aliases:   SID6-1512
RefSeq:   NC_000009.11 NT_008470.19
Ensembl:   ENSG00000119408
Entrez:   10783
Chromosome Mapping:   9q33.3-q34.11

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 35.750000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download