PrimePCR™ SYBR® Green Assay: PCDH17, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0051058

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0043796
Assay Design:   exonic
Chromosome Location:   13:58299700-58299829question
Amplicon Length:   100
Splice Variants Targeted:   ENST00000377918

Gene Information

This gene belongs to the protocadherin gene family a subfamily of the cadherin superfamily. The encoded protein contains six extracellular cadherin domains a transmembrane domain and a cytoplasmic tail differing from those of the classical cadherins. The encoded protein may play a role in the establishment and function of specific cell-cell connections in the brain. [provided by RefSeq Jul 2008]

Gene Symbol:   PCDH17
Gene Name:   protocadherin 17
Aliases:   PCDH68, PCH68
RefSeq:   NC_000013.10 NT_024524.14
Ensembl:   ENSG00000118946
Entrez:   27253
UniGene:   Hs.106511
Chromosome Mapping:   13q21.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 35.520000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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