PrimePCR™ SYBR® Green Assay: SGK1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0051045

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: SGK1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: SGK1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0043783
Assay Design:   exonic
Chromosome Location:   6:134494695-134495660question
Amplicon Length:   68
Splice Variants Targeted:   ENST00000367858 ENST00000413996 ENST00000237305 ENST00000367857 ENST00000528577 ENST00000475719 ENST00000461976

Gene Information

This gene encodes a serine/threonine protein kinase that plays an important role in cellular stress response. This kinase activates certain potassium sodium and chloride channels suggesting an involvement in the regulation of processes such as cell survival neuronal excitability and renal sodium excretion. High levels of expression of this gene may contribute to conditions such as hypertension and diabetic nephropathy. Several alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq Jan 2009]

Gene Symbol:   SGK1
Gene Name:   serum/glucocorticoid regulated kinase 1
Aliases:   SGK
RefSeq:   NC_000006.11 NT_025741.15
Ensembl:   ENSG00000118515
Entrez:   6446
Chromosome Mapping:   6q23

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 36.530000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download