PrimePCR™ SYBR® Green Assay: ALDH8A1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0051044

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0043782
Assay Design:   exonic
Chromosome Location:   6:135239667-135239795question
Amplicon Length:   99
Splice Variants Targeted:   ENST00000265605 ENST00000367845 ENST00000367847 ENST00000460753

Gene Information

This protein belongs to the aldehyde dehydrogenases family of proteins. It plays a role in a pathway of 9-cis-retinoic acid biosynthesis in vivo. This enzyme converts 9-cis-retinal into the retinoid X receptor ligand 9-cis-retinoic acid and has approximately 40-fold higher activity with 9-cis-retinal than with all-trans-retinal. Therefore it is the first known aldehyde dehydrogenase to show a preference for 9-cis-retinal relative to all-trans-retinal. Three transcript variants encoding distinct protein isoforms have been identified for this gene. [provided by RefSeq Jul 2010]

Gene Symbol:   ALDH8A1
Gene Name:   aldehyde dehydrogenase 8 family, member A1
Aliases:   ALDH12, DJ352A20.2, DKFZp779D2315, MGC138650
RefSeq:   NC_000006.11 NT_025741.15 NG_012261.1
Ensembl:   ENSG00000118514
Entrez:   64577
UniGene:   Hs.486520
Chromosome Mapping:   6q23.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 36.520000
Efficiency 93

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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