PrimePCR™ SYBR® Green Assay: STAG1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0051023

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: STAG1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: STAG1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0043761
Assay Design:   exonic
Chromosome Location:   3:136162141-136170881question
Amplicon Length:   83
Splice Variants Targeted:   ENST00000383202 ENST00000236698 ENST00000492318 ENST00000434713 ENST00000536929

Gene Information

This gene is a member of the SCC3 family and is expressed in the nucleus. It encodes a component of cohesin a multisubunit protein complex that provides sister chromatid cohesion along the length of a chromosome from DNA replication through prophase and prometaphase after which it is dissociated in preparation for segregation during anaphase. [provided by RefSeq Jul 2008]

Gene Symbol:   STAG1
Gene Name:   stromal antigen 1
Aliases:   DKFZp781D1416, SA1
RefSeq:   NC_000003.11 NT_005612.16
Ensembl:   ENSG00000118007
Entrez:   10274
Chromosome Mapping:   3q22.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999000
y-intercept 35.070000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download