PrimePCR™ SYBR® Green Assay: CTSD, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0051022

List Price:    $138.00
Your Price:   Log In
 
Add to Custom Plate

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0043760
Assay Design:   exonic
Chromosome Location:   11:1780239-1780762question
Amplicon Length:   66
Splice Variants Targeted:   ENST00000367196 ENST00000438213 ENST00000236671

Gene Information

This gene encodes a lysosomal aspartyl protease composed of a dimer of disulfide-linked heavy and light chains both produced from a single protein precursor. This proteinase which is a member of the peptidase C1 family has a specificity similar to but narrower than that of pepsin A. Transcription of this gene is initiated from several sites including one which is a start site for an estrogen-regulated transcript. Mutations in this gene are involved in the pathogenesis of several diseases including breast cancer and possibly Alzheimer disease. [provided by RefSeq Jul 2008]

Gene Symbol:   CTSD
Gene Name:   cathepsin D
Aliases:   CLN10, CPSD, MGC2311
RefSeq:   NC_000011.9 NG_008655.1 NT_009237.18
Ensembl:   ENSG00000117984
Entrez:   1509
UniGene:   Hs.654447
Chromosome Mapping:   11p15.5

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.920000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download