PrimePCR™ SYBR® Green Assay: SLC35A3, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0051009

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0043747
Assay Design:   exonic
Chromosome Location:   1:100459105-100459224question
Amplicon Length:   90
Splice Variants Targeted:   ENST00000370155 ENST00000465289 ENST00000427993 ENST00000532693 ENST00000422078 ENST00000370153

Gene Information

Description Not Available

Gene Symbol:   SLC35A3
Gene Name:   solute carrier family 35 (UDP-N-acetylglucosamine (UDP-GlcNAc) transporter), member A3
Aliases:   DKFZp781P1297
RefSeq:   NC_000001.10 NT_032977.9
Ensembl:   ENSG00000117620
Entrez:   23443
UniGene:   Hs.448979
Chromosome Mapping:   1p21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.320000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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