PrimePCR™ SYBR® Green Assay: KDM5B, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0050985

List Price:    $142.00
Your Price:   Log In
 
Add to Custom Plate

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0043723
Assay Design:   exonic
Chromosome Location:   1:202718141-202719790question
Amplicon Length:   101
Splice Variants Targeted:   ENST00000367265 ENST00000367264 ENST00000235790

Gene Information

Description Not Available

Gene Symbol:   KDM5B
Gene Name:   lysine (K)-specific demethylase 5B
Aliases:   CT31, FLJ10538, FLJ12459, FLJ12491, FLJ16281, FLJ23670, JARID1B, PLU-1, PLU1, PUT1, RBBP2H1A
RefSeq:   NC_000001.10 NT_004487.19
Ensembl:   ENSG00000117139
Entrez:   10765
UniGene:   Hs.443650
Chromosome Mapping:   1q32.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 35.230000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download